Credentials: Biochemistry Department
Position title: Mechanisms of pre-mRNA Splicing
Phone: (608) 890-3101
433 Babcock Dr
Madison, WI 53706
RNA Biology; Molecular and Genome Biology of Microbes
My laboratory is interested in mechanisms of pre-mRNA splicing in eukaryotes, and we study this problem using a combination of single molecule fluorescence microscopy, yeast genetics, ensemble enzyme assays, and chemical biology. I am particularly interested in how the spliceosome identifies splice sites (the locations of bond cleavage and formation) in pre-mRNA transcripts. Correct identification of these sites is essential for splicing fidelity, and the spliceosome relies on a network of cooperative interactions between RNAs and proteins to identify introns. Using a single molecule approach, we can quantitatively analyze how spliceosomes interact with pre-mRNA. These experiments are focused on elucidating mechanisms of spliceosomal U1 snRNP interaction with the 5’ splice site, the role auxiliary splicing proteins play in regulated and alternative splicing in yeast and other organisms, and how ATP hydrolysis is used to proofread the interaction between the U2 snRNP and the pre-mRNA branchsite.