Amy Weeks
Credentials: Biochemistry Department
Position title: Spatiotemporal dynamics of cell signaling using chemical biology and proteomics tools
Email: amweeks@wisc.edu
Phone: 608-890-2583
Address:
6426D Hector F. DeLuca Biochemical Sciences Building
440 Henry Mall
Madison, WI 53706
LAB WEBSITE:
FOCUS GROUPS:
Systems Biology, Membrane Biology & Protein Trafficking, Cancer Biology
RESEARCH DESCRIPTION:
Eukaryotic signaling pathways rely on the introduction of PTMs to specific proteins to rapidly change protein function and localization, enabling cells to respond to changing internal or environmental conditions. However, despite the importance of spatial organization and temporal dynamics in biological signaling, current technologies are unable to provide a
systems-level experimental mapping of the dynamic subcellular localization of post-translationally modified proteins. To meet this challenge, we are developing new methods for PTM proteomics with subcellular spatial and temporal resolution by engineering genetically targetable, PTM-selective proximity labeling enzymes. These enzymes tag post-translationally modified proteins in specific subcellular locations, enabling their enrichment and analysis with mass spectrometry-based proteomics experiments for mapping PTMs with spatial and temporal resolution. Using these tools, we seek to provide an integrated view of cellular signaling to advance biological discovery, with potential therapeutic implications.
ALSO A TRAINER IN THE FOLLOWING PROGRAMS:
Chemistry, Integrated Program in Biochemistry (IpiB), Biophysics